At5g67030(ZEP) ORF Clone

产品内容

pLinker1-At5g67030------------------------2 μg

TE buffer--------------------------------------500 μl

产品信息

产品名称：At5g67030(ZEP) ORF Clone

产品货号：QA5g67030

别称：ZEP;ABA1;IBS3;LOS6;NPQ2;At5g67030;K8A10.10

产品类型：ORF克隆载体

基因ID：At5g67030

转录本：AT5G67030.1

载体：pLinker1

抗性：kanR

ORF 长度：2004 bp

蛋白长度：667 aa

蛋白分子量：73842 Da

片段物种：拟南芥（Arabidopsis thaliana）

NCBI gene ID：836838

Uniprot ID：Q9FGC7

基因摘要

Encodes a single copy zeaxanthin epoxidase gene that functions in first step of the biosynthesis of the abiotic stress hormone abscisic acid (ABA). Mutants in this gene are unable to express female sterility in response to beta-aminobutyric acid, as wild type plants do.

ORF 核苷酸序列

>At5g67030(ZEP) ORF Clone

ACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCCAGTGAATTCGAGCTCGGTACCTCGCGAATGCATCTAGATATC

ATGGGTTCAACTCCGTTTTGCTACTCTATCAATCCATCTCCATCAAAGCTTGATTTCACGAGGACCCATGTGTTTAGTCCTGTTTCTAAACAGTTTTACTTAGATTTATCATCGTTTTCCGGAAAACCCGGAGGAGTATCTGGGTTTAGGAGCCGTCGAGCTTTGCTCGGAGTAAAGGCGGCGACGGCGTTAGTTGAGAAGGAGGAGAAGAGAGAGGCGGTGACGGAGAAGAAGAAGAAATCGAGGGTTTTAGTTGCCGGAGGTGGAATCGGAGGATTGGTGTTTGCTTTAGCGGCTAAGAAGAAAGGATTCGATGTGTTAGTGTTTGAGAAAGATTTGAGTGCTATAAGAGGAGAAGGAAAATACAGAGGCCCGATTCAAATACAGAGCAACGCTTTAGCTGCTTTGGAAGCTATTGATATTGAAGTTGCTGAACAAGTTATGGAAGCTGGGTGTATCACTGGTGATCGGATTAACGGTCTCGTTGATGGTATCTCTGGTACTTGGTATGTAAAGTTTGATACTTTCACTCCTGCGGCGTCACGGGGACTTCCTGTGACTAGAGTAATTAGTAGAATGACTCTGCAGCAGATTCTAGCACGTGCGGTTGGAGAAGATGTGATTAGAAACGAGAGTAATGTTGTTGATTTTGAAGATTCTGGAGATAAGGTTACTGTGGTACTCGAGAATGGTCAACGCTATGAAGGTGATCTGCTTGTGGGTGCAGATGGCATTTGGTCTAAGGTGAGAAATAATTTGTTTGGCCGTAGTGAAGCTACTTATTCAGGCTACACTTGTTACACGGGGATTGCAGATTTTATACCAGCGGATATCGAGTCTGTTGGCTACCGGGTTTTCTTGGGACACAAACAGTACTTTGTTTCTTCGGATGTTGGTGGTGGAAAAATGCAATGGTATGCATTTCACGAGGAACCAGCTGGTGGGGCTGATGCTCCAAATGGTATGAAGAAAAGGTTGTTTGAAATATTTGACGGTTGGTGCGACAATGTACTCGACTTGTTGCATGCGACTGAGGAGGAAGCCATTCTGAGAAGAGATATTTATGATAGAAGTCCTGGTTTTACTTGGGGTAAAGGGCGTGTTACGCTGCTCGGGGATTCTATCCATGCGATGCAGCCAAATATGGGTCAAGGTGGATGCATGGCCATTGAGGATAGTTTTCAACTAGCATTGGAGCTTGATGAAGCATGGAAACAGAGTGTTGAAACGACTACACCTGTTGATGTTGTTTCCTCTTTGAAAAGATATGAGGAATCTAGAAGACTGAGAGTCGCTATTATCCATGCAATGGCGAGGATGGCTGCAATTATGGCTTCCACTTACAAAGCATACTTAGGTGTTGGGCTTGGTCCTCTGTCTTTCTTGACAAAGTTTAGAGTACCACATCCAGGAAGAGTTGGTGGTAGATTCTTCGTTGACATTGCTATGCCATCGATGCTTGACTGGGTCCTTGGAGGTAACAGTGAAAAACTCCAAGGAAGGCCACCTAGTTGCAGACTCACTGACAAAGCCGATGACCGGCTTCGAGAGTGGTTTGAAGATGACGATGCTCTTGAACGTACTATAAAGGGAGAATGGTATCTAATTCCACACGGCGACGATTGTTGCGTTTCGGAAACATTATGTCTAACCAAAGATGAAGATCAACCTTGCATCGTCGGAAGCGAACCAGATCAAGATTTTCCTGGAATGCGCATTGTGATCCCTTCGTCTCAGGTTTCGAAGATGCATGCTCGTGTGATTTACAAAGACGGAGCTTTCTTCTTGATGGATCTTCGAAGCGAACACGGAACCTATGTGACCGATAACGAAGGAAGAAGATATAGAGCAACACCGAATTTTCCCGCGCGGTTTAGATCGTCCGACATCATCGAGTTTGGTTCAGATAAGAAGGCGGCGTTTAGGGTGAAAGTAATCAGGAAAACTCCGAAATCGACGAGGAAGAATGAGAGTAACAACGATAAATTACTTCAGACAGCTTGA

GATATCGGATCCCGGGCCCGTCGACTGCAGAGGCCTGCATGCAAGCTTGGCGTAATCATGGTCATACCCTATAGTGAGTCGTATTACATGGTCATAGCTG

黑色：载体序列、红色：同源重组序列、绿色：EcoR V酶切位点、蓝色：ORF序列

蛋白简介

Zeaxanthin epoxidase that plays an important role in the xanthophyll cycle and abscisic acid (ABA) biosynthesis. Converts zeaxanthin into antheraxanthin and subsequently violaxanthin. Required for resistance to osmotic and drought stresses, ABA-dependent stomatal closure, seed development and dormancy, modulation of defense gene expression and disease resistance and non-photochemical quencing (NPQ). Through its role in ABA biosynthesis, regulates the expression of stress-responsive genes such as RD29A during osmotic stress and is required for normal plant growth during vegetative development. Is required for late skotomorphogenic growth through its role in the xanthophyll carotenoids neoxanthin, violaxanthin and antheraxanthin biosynthesis. Required for beta-aminobutyric acid (BABA)-induced priming in disease resistance, tolerance to salt and drought stresses and sterility. Participates in NPQ by regulating the level of zeaxanthin in photosynthetic energy conversion. NPQ is a process that maintains the balance between dissipation and utilization of light energy to minimize the generation of oxidizing molecules and the molecular damages they can generate. {ECO:0000269|PubMed:11779861, ECO:0000269|PubMed:12447533, ECO:0000269|PubMed:14722117, ECO:0000269|PubMed:15053765, ECO:0000269|PubMed:15548743, ECO:0000269|PubMed:15722464, ECO:0000269|PubMed:15983017, ECO:0000269|PubMed:16113213, ECO:0000269|PubMed:17416642, ECO:0000269|PubMed:17996011, ECO:0000269|PubMed:18680727, ECO:0000269|PubMed:19278749, ECO:0000269|PubMed:9668132}.

蛋白质序列

>Zeaxanthin epoxidase, chloroplastic (AtZEP) (EC 1.14.15.21) (Protein ABA DEFICIENT 1) (AtABA1) (Protein IMPAIRED IN BABA-INDUCED STERILITY 3) (Protein LOW EXPRESSION OF OSMOTIC STRESS-RESPONSIVE GENES 6) (Protein NON-PHOTOCHEMICAL QUENCHING 2)

MGSTPFCYSINPSPSKLDFTRTHVFSPVSKQFYLDLSSFSGKPGGVSGFRSRRALLGVKAATALVEKEEKREAVTEKKKKSRVLVAGGGIGGLVFALAAKKKGFDVLVFEKDLSAIRGEGKYRGPIQIQSNALAALEAIDIEVAEQVMEAGCITGDRINGLVDGISGTWYVKFDTFTPAASRGLPVTRVISRMTLQQILARAVGEDVIRNESNVVDFEDSGDKVTVVLENGQRYEGDLLVGADGIWSKVRNNLFGRSEATYSGYTCYTGIADFIPADIESVGYRVFLGHKQYFVSSDVGGGKMQWYAFHEEPAGGADAPNGMKKRLFEIFDGWCDNVLDLLHATEEEAILRRDIYDRSPGFTWGKGRVTLLGDSIHAMQPNMGQGGCMAIEDSFQLALELDEAWKQSVETTTPVDVVSSLKRYEESRRLRVAIIHAMARMAAIMASTYKAYLGVGLGPLSFLTKFRVPHPGRVGGRFFVDIAMPSMLDWVLGGNSEKLQGRPPSCRLTDKADDRLREWFEDDDALERTIKGEWYLIPHGDDCCVSETLCLTKDEDQPCIVGSEPDQDFPGMRIVIPSSQVSKMHARVIYKDGAFFLMDLRSEHGTYVTDNEGRRYRATPNFPARFRSSDIIEFGSDKKAAFRVKVIRKTPKSTRKNESNNDKLLQTA

关键词

Abscisic acid biosynthesis;Alternative splicing;Chloroplast;FAD;Flavoprotein;Oxidoreductase;Plastid;Reference proteome;Stress response;Transit peptide


质粒图谱

产品说明

本产品是经过离子交换柱纯化后，装在无菌冻存管中通过真空干燥获得的2 μg连有At5g67030(ZEP) 基因ORF的质粒DNA干粉。

保存：-20 ℃

使用说明

1、以5000 × g离心5 min；

2、打开冻存管，加入20 μL无菌水溶解DNA，并在室温下孵育10 min；

3、短暂涡旋冻存管，然后瞬时离心 (小于5000 × g)。

4、质粒溶液在-20℃下可稳定保存一年，请避免反复冻融。


扩增方案

1、收到质粒干粉后请先5000 rpm离心1 min，再加入20 μL无菌水溶解质粒，室温放置1 min；

2、从-80 °C冰箱中取出相应的感受态，置于冰盒上解冻，并做好标记；

3、取2 μL质粒加至100 μL感受态中，冰浴30min;

4、42 °C热激90 s，再冰浴2 min;

5、加入900 μL无抗的LB液体培养基，200 rpm震荡培养45 min;

6、5000 rpm离心5 min，仅留100 μL上清混匀菌体沉淀；

7、混匀后的菌液加至对应抗性的LB平板上，倒入适量玻璃珠，涂匀液体；

8、将平板正向培养1 h，再倒置培养12 h～16 h;

9、挑取单克隆菌落至对应抗性的LB液体培养基中，震荡培养12 h~16 h，根据实验需要提取质粒。

注意事项

1、同一个基因的单个转录序列可能由于发生自然变异 (例如核苷酸多态性)而有所不同，每一种都有其自身有效的存在。

2、该克隆被工程化，但表达取决于基因的性质。

3、本产品仅限于科学研究，不得用于临床诊断或治疗，不得用于食品或药品。

4、为了您的安全和健康，请穿实验服并戴一次性手套操作。

Tair 网站：https://www.arabidopsis.org/servlets/Search?type=general&search_action=detail&method=1&show_obsolete=F&name=At5g67030&sub_type=gene&SEARCH_EXACT=4&SEARCH_CONTAINS=1
NCBI网站：https://www.ncbi.nlm.nih.gov/gene/?term=836838
Uniprot 网站：https://www.uniprot.org/uniprotkb//entry

相关文献 (NCBI PubMed ID)

PMID: 10859185; 27862469; 14593172; 16666794; 9668132; 11124119; 11707572; 11779861; 12447533; 12047634; 14722117; 15548743; 15053765; 15983017; 15722464; 16113213; 17416642; 18680727; 17996011; 19278749